Coding
bbp9

Part:BBa_K732003:Design

Designed by: Joshua Fass   Group: iGEM12_VGEM   (2012-10-01)


bbp9 - Bordetella phage lysin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 910
    Illegal NgoMIV site found at 1290
    Illegal NgoMIV site found at 1315
    Illegal NgoMIV site found at 1407
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Isolated from phage genome, made BioBrick-compatible.


Source

PCR-amplification from isolated BPP phage genome. Described as a putative lysin in: M Liu, M Gingery, SR Doulatov, Y Liu, A Hodes, S Baker, P Davis, M Simmonds, C Churcher, K Mungall, MA Quail, A Preston, ET Harvill, DJ Maskell, FA Eiserling, J Parkhill, JF Miller. (2004). Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes. Journal of Bacteriology 186(5):1503.

References